adenovector mediated inflammation in mice liver boosted by hydrodynamic injection

background and aims: adenovector gene transfer induces inflammatory response that finally leads to vector removal from delivered site. the effect of hydrodynamic pre-injection on adenovector mediated liver inflammation remained elusive. materials and methods: different mice groups were pre-treated by hydrodynamic saline, dexamethasone or nothing prior to adeno-luciferase administration. their samples were collected in 18 hours later and serum il-1β level as a marker of inflammation was quantified. also, liver histological assessment was performed to score the inflammatory grade. results and conclusion: in spite of dexamethasone group, both hydrodynamic/ad-luc and ad-luc receiving groups induced the expression of il-1β cytokine (p<0.05). the pathological views also indicated more inflammation and necrosis inside the different zones of liver tissue in these two adenovector injected groups. these results emphasized that adenovector served as a suitable inflammatory model and hydrodynamic injection enhances this kind of inflammation.